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| Comparison of Extraction Methods for the Determination of Vitamin E in Some Grains |
| Seon⁃Mi Lee, Hee⁃Bong Lee and Junsoo Lee |
| Dept. of Food and Science Technology, Chungbuk National University, Cheongju 361-763, Korea |
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Abstract
| For the determination of vitamin E using high performance liquid chromatography (HPLC), the most critical and time consuming step is the quantitative extraction of all vitamin E homologs from sample matrix. Three different extraction methods were compared to determine vitamin E in grains. Saponification used alkaline hydrolysis followed by solvent extraction. Direct solvent extraction included dispersing the samples in hot water, addition of isopropanol and MgSO4, and extraction of the analytes with extracting solvent with Polytron homogenization. Using Soxhlet extraction, the samples were extracted in a Soxhlet apparatus with hexane containing butylated hydroxytoluene (BHT). Vitamin E content in 14 grains were analyzed and compared by three different extraction methods. Generally, the highest values were observed from direct solvent extraction and Soxhlet extraction followed by saponification. α⁃Tocopherol equivalent (α⁃TE) levels in grains ranged from 0.32 α⁃TE/100 g in prosomillet to 5.12 α⁃TE/100 g in black rice. |
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| Key words : grams:vitamin E:saponification:direct solvent extraction:Soxhlet extraction |
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| Journal of the Korean Society of Food Science and Nutrition 35(2), 248~253 (2006) |
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