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| Purification and Characterization of the Extracellular Alginase Produced by Bacillus licheniformis AL⁃577 |
| Meung⁃Hee Uo1, Dong⁃Sik Joo2, Soon⁃Yeong Cho3 and Tae⁃Sun Min4 |
| 1Marine Bioindustry Course, Graduate School, Kangnung National University, Kangnung 210-702, Korea; 2Division of Oriental Well-being, Hanzhong University, Donghae 240-713, Korea; 3East Coastal Marine Bioresources Research Center, Kangnung National University, Kangnung 210-702, Korea; 4Korea Science and Engineering Foundation, Daejeon 305-350, Korea |
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Abstract
| The extracellular enzyme alginase produced by Bacillus licheniformis AL⁃577 was purified by ion chro⁃matography on CM⁃Cellulose column, DEAE⁃Sepharose column, and followed by gel filtration on Sephadx G⁃100 column. The optimum pH and temperature for the activity of the purified enzyme were 6.0 and 35oC, respectively. The enzyme was stable at the pH range of 6.0～9.0 and at 20oC. The molecular weight of the enzyme was estimated to be about 25,500 daltons by SDS⁃polyacrylamide gel electrophoresis. NaCl was required for high activity of the enzyme. The enzyme was inhibited by Ba2+, Co2+, Cu2+, Fe2+, Mg2+, Zn2+, NH4+, EDTA, L⁃cysteine, and 2⁃mercaptoethanol, while stimulated by DTT, O⁃phenanthroline, K+ and Li+. This enzyme was proposed to be an alginase specifically degrading alginic acid. |
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| Key words : alginase:extracellular enzyme:alginic acid:Bacillus licheniformis |
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| Journal of the Korean Society of Food Science and Nutrition 35(2), 231~237 (2006) |
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